Clonal types and antimicrobial resistance profiles of methicillin-resistant Staphylococcus aureus isolates from hospitals in south Brazil.
نویسندگان
چکیده
In the present study were evaluated the DNA macrorestriction profile and SCCmec types for nine multi-resistant MRSA selected. Also antimicrobial susceptibility testing by disk diffusion method was evaluated for 68 MRSA isolates against 12 antimicrobial agents. The isolates were recovered from blood culture collected from hospitalized patients in three hospitals of Porto Alegre, Brazil. PFGE and PCR for mecA and SCCmec I, II, III, IV types genes were done on selected nine isolates with susceptibility only to vancomycin, teicoplanin and linezolid. Two clone profiles, with five subtypes, were demonstrated among multi-resistant MRSA analyzed. Eight isolates showed harbor SCCmec type III and one isolate was not typeable. The knowledge of SCCmec type, clone and antimicrobial profiles among S. aureus is essential mainly to prevention and control of dissemination of the antimicrobial resistance.
منابع مشابه
Detection of Methicillin-resistant Staphylococcus aureus according to mecA and femA Genes Among Hospitalized Patients in Kashan and Isfahan Hospitals
Background and Aim: Increased prevalence of methicillin-resistant Staphylococcus aureus (MRSA) and treatment of infections due to these resistant strains is one of the most important health challenges. In this study, antimicrobial resistance patterns of Staphylococcus aureus isolates from patients were determined to commonly prescribed and last-resort antibiotics, and methicillin-resistant Stap...
متن کاملComplete substitution of the Brazilian endemic clone by other methicillin-resistant Staphylococcus aureus lineages in two public hospitals in Rio de Janeiro, Brazil.
Staphylococcus aureus is an important cause of bloodstream infections. Therefore, the main purpose of this work was to characterize a collection of 139 S. aureus isolates from bloodstream infections in two public hospitals in relation to their antimicrobial susceptibility profile, staphylococcal cassette chromosome mec types, and clonal relationship. Methicillin resistance and resistance to oth...
متن کاملAssociation between biofilm production, adhesion genes and drugs resistance in different SCCmec types of methicillin resistant Staphylococcus aureus strains isolated from several major hospitals of Iran
Objective(s): The ability of bacteria to produce biofilm and adhesion makes them more resistant to antibiotics. The current study aims to evaluate the biofilm formation by Staphylococcus aureus and to determine the prevalence of adhesion genes, also their correlation with drug resistance.Materials and Methods: A total of 96 MRSA were collected from hospitals of Iran’s western provinces during 2...
متن کاملerm(C) is the predominant genetic determinant for the expression of resistance to macrolides among methicillin-resistant Staphylococcus aureus clinical isolates in Greece.
OBJECTIVES Macrolide, lincosamide and streptogramin type B (MLS(B)) resistance was determined in Staphylococcus aureus clinical isolates from two University Hospitals. METHODS Antibiotic resistance was investigated by double disc diffusion and MIC determination. Resistance determinants were detected by PCR and DNA hybridization, while clonal types were identified by pulsed-field gel electroph...
متن کاملIsolation, antimicrobial susceptibility and mecA gene analysis of methicillin-resistant Staphylococcus aureus
The objective of this study was to determine the occurrence of antimicrobial resistance among Staphylococcus aureus and to estimate the presence of methicillin-resistance in S. aureus (MRSA) isolates obtained by culture and polymerase chain reaction (PCR) methods. For this purposes, 100 Iranian white and feta cheese samples collected from different suppliers were initially evaluated for the occ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Revista do Instituto de Medicina Tropical de Sao Paulo
دوره 50 3 شماره
صفحات -
تاریخ انتشار 2008